Anti-MRPL21 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q7Z2W9

Core Product Specifications and Parameters

Biological Background: MRPL21 Function and Localization

• MRPL21 encodes the mitochondrial large ribosomal subunit protein bL21m, also known as 39S ribosomal protein L21 (MRP-L21/L21mt).
• As a component of the 39S mitoribosomal subunit, it is predicted to participate in the translation of the 13 essential proteins of the oxidative phosphorylation machinery encoded by the mitochondrial genome.
• The protein localizes exclusively to the mitochondrion, consistent with its role in mitochondrial protein synthesis, and contains a transit peptide for mitochondrial targeting.
• Alternative splicing of MRPL21 has been reported, potentially generating multiple transcript variants, though their functional significance remains to be fully characterized.
• Structural analyses of the human mitoribosome, including cryo-electron microscopy studies, have revealed the positioning and interactions of bL21m within the large subunit.
• MRPL21 has been identified in proteomics screens and mass spectrometry-based studies, further confirming its expression at the protein level.
• Key functional keywords from UniProt include ribonucleoprotein, ribosomal protein, mitochondrion, alternative splicing, and transit peptide.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 40–140 of human MRPL21; this region may be conserved in common isoforms and could detect both full-length and alternatively spliced products.
• Western blot analysis typically yields bands around 13 kDa and 22 kDa, likely corresponding to different isoforms or processing forms. Consider including mitochondrial-enriched lysates to enhance signal specificity.
• For immunofluorescence applications (IF/ICC, IF-P), signal is expected in mitochondrial structures; co-staining with mitochondrial markers may aid in confirming localization.
• This antibody is validated for multiple applications: WB, IF/ICC, IF-P, IHC-P, and ELISA. Optimal working dilutions and conditions should be determined empirically in your experimental system.
• When using for immunohistochemistry (IHC-P), perform appropriate antigen retrieval and validate staining patterns in control tissues with known mitochondrial content.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated supply solutions for IVD raw materials, offering this anti-MRPL21 monoclonal antibody along with validated antibody pairs, bulk ancillary reagents, and sourcing support for mitochondrial research targets. Whether you require custom rabbit monoclonal antibodies, rare target procurement, or technical consulting from concept to clinic, CamelBio streamlines procurement and delivers application-driven service for diagnostic manufacturers and research laboratories worldwide.