Anti-MRPL20 Polyclonal Antibody for WB and ELISA - Q9BYC9

Core Product Specifications and Parameters

Biological Background: MRPL20 Function and Localization

• MRPL20 encodes a protein of the mitochondrial large ribosomal subunit, also known as 39S ribosomal protein L20, mitochondrial (L20mt or MRP-L20).
• The protein is a structural constituent of the mitochondrial ribosome, contributing to the assembly and function of the 39S subunit.
• It belongs to the ribosomal protein bL20 family, which is conserved across prokaryotes and eukaryotic mitochondria.
• Subcellular localization: MRPL20 is targeted to the mitochondrion, where it functions (as indicated by its transit peptide and confirmed by proteomics).
• According to UniProt keywords, alternative splicing generates multiple transcript variants, potentially yielding isoforms with distinct properties.
• The protein has been identified in high-throughput proteomic studies, supporting its expression in various human tissues.
• Structural studies (3D-structure keyword) have characterized MRPL20 within the mitochondrial ribosome, providing insights into its role in protein synthesis.
• The molecular weight of the canonical isoform is approximately 17.4 kDa (UniProt), aligning with the observed 17 kDa band.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50–149 of human MRPL20 (NP_060441.2). This region is likely exposed and immunogenic.
• For Western blot, consider using mitochondrial-enriched lysates to improve detection, as MRPL20 is a mitochondrial protein. A band at ~17 kDa is expected.
• When performing ELISA, titrate the antibody to optimize signal-to-noise ratio, and include appropriate positive and negative controls.
• Cross-reactivity is reported for human, mouse, and rat. However, sequence homology in the immunogen region should be verified if using other species.
• The antibody is supplied as a polyclonal rabbit IgG. For reproducibility, aliquot and store at -20°C or as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with seamless access to high-quality IVD raw materials. This anti-MRPL20 polyclonal antibody is part of our extensive catalog of mitochondrial research tools, supporting studies in oxidative phosphorylation and mitochondrial diseases. Through our global network, we also offer validated antibody pairs, recombinant proteins, and custom sourcing for rare targets to accelerate your assay development.