Anti-Mrpl18 Rabbit Polyclonal Antibody for WB & ELISA - Q9CQL5

Core Product Specifications and Parameters

Biological Background: Mrpl18 Function and Localization

• Mrpl18, also known as 39S ribosomal protein L18, mitochondrial (MRP-L18) or Large ribosomal subunit protein uL18m, is a component of the mitochondrial ribosome.
• It contains a transit peptide and localizes to the mitochondrion.
• Mrpl18 functions together with thiosulfate sulfurtransferase (TST) as a mitochondrial import factor for the cytosolic 5S rRNA.
• The precursor form of Mrpl18 exhibits RNA chaperone activity, folding the 5S rRNA into an import-competent conformation recognized by rhodanese (TST).
• Both cytoplasmic and mitochondrial forms of Mrpl18 bind to the helix IV-loop D in the gamma domain of the 5S rRNA.
• Relevant keywords include: Chaperone, Ribonucleoprotein, Ribosomal protein, RNA-binding, Transit peptide, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 42–180 of mouse Mrpl18, which may preserve native epitopes; empirical testing is recommended to confirm performance in denaturing vs. native conditions.
• For Western blot (WB), consider using mitochondrial-enriched fractions from human or mouse cells to enhance signal detection, as Mrpl18 is a mitochondrial protein.
• The antibody cross-reacts with both human and mouse Mrpl18, making it suitable for studies across these species; validate in the relevant sample system first.
• In ELISA applications, optimization of coating antigen concentration and detection conditions is advised to maximize sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Mrpl18 antibody is part of our extensive portfolio for mitochondrial and ribosomal protein research. Our supply capabilities include validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets to accelerate your assay development and production.