Anti-MRPL18 Polyclonal Antibody for WB, IF, ELISA - Q9H0U6

Core Product Specifications and Parameters

Biological Background: MRPL18 Function and Localization

• MRPL18 encodes the mitochondrial large ribosomal subunit protein uL18m (also known as 39S ribosomal protein L18, mitochondrial).
• The protein is essential for mitochondrial ribosome biogenesis and function.
• Beyond its ribosomal role, MRPL18 acts together with thiosulfate sulfurtransferase (TST) to facilitate the import of cytosolic 5S rRNA into mitochondria.
• The precursor form of MRPL18 displays RNA chaperone activity, assisting in folding 5S rRNA into an import-competent conformation recognized by TST.
• Both cytoplasmic and mitochondrial isoforms of MRPL18 bind to the helix IV-loop D region within the gamma domain of the 5S rRNA.
• MRPL18 is predominantly localized to the mitochondrion, consistent with its roles in mitochondrial translation and RNA import.
• UniProt keywords: Chaperone, Mitochondrion, Ribonucleoprotein, Ribosomal protein, RNA-binding, Transit peptide, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 92 amino acids of human MRPL18, which are present in both the precursor and mature forms. This design may allow detection of the full-length precursor and processed protein in mitochondrial extracts.
• For Western blot, consider using mitochondrial-enriched lysates to enhance signal and validate band specificity with knockdown or knockout controls.
• In immunofluorescence (IF/ICC), co-staining with mitochondrial markers (e.g., Tom20, COX IV) is recommended to confirm mitochondrial localization.
• For ELISA applications, titrate the antibody concentration and optimize the detection system using recombinant MRPL18 as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing hub for IVD raw materials, supporting diagnostic manufacturers and research institutes with high-quality reagents from discovery through clinical development. Our portfolio includes validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents tailored for mitochondrial and ribosomal targets. For MRPL18 and related research areas, we facilitate procurement of rare-target antibodies, recombinant proteins, and technical consulting to accelerate your assay development and biomarker studies.