Anti-MRPL16 Polyclonal Antibody for WB, ELISA - Q9NX20

Core Product Specifications and Parameters

Biological Background: MRPL16 Function and Localization

• MRPL16, also known as uL16m or 39S ribosomal protein L16, is a component of the large subunit of the mitochondrial ribosome.
• The protein is encoded by the MRPL16 gene (GeneID: 54948) and is a key ribosomal protein involved in mitochondrial protein synthesis.
• Alternative names include L16mt, MRP-L16, and PNAS-111, reflecting its mitochondrial localization and historical designations.
• Immunofluorescence and biochemical studies indicate that MRPL16 localizes exclusively to the mitochondria, consistent with its role in the mitochondrial ribosome.
• As a ribosomal protein, MRPL16 is part of the ribonucleoprotein complex and is essential for mitochondrial translation.
• The protein is classified under the keywords: 3D-structure, mitochondrion, proteomics identification, reference proteome, ribonucleoprotein, ribosomal protein, and transit peptide.
• Its molecular weight is approximately 28kDa, matching the predicted size of the mature protein after cleavage of its mitochondrial targeting sequence.
• MRPL16 is conserved across species, with cross-reactivity observed in human, mouse, and rat, facilitating comparative studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100), which may target conserved epitopes. Verify specificity in the relevant sample matrix.
• For Western blotting, a band around 28kDa is expected in lysates from human, mouse, or rat mitochondria-enriched fractions.
• Consider optimizing antibody dilution for each assay; typical starting dilutions for polyclonal antibodies range from 1:500 to 1:2000 for WB and 1:1000 to 1:5000 for ELISA.
• Employ appropriate protein extraction methods that preserve mitochondrial integrity to enhance detection.
• Use protease inhibitors during lysis to prevent degradation of this mitochondrial protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. This anti-MRPL16 antibody supports mitochondrial research and ribosome-related studies; we also provide customized sourcing for rare targets and technical consulting to streamline your assay development. Let CamelBio bridge your supply needs from concept to clinic.