Anti-MRPL12 Rabbit pAb for WB - P52815

Core Product Specifications and Parameters

Biological Background: MRPL12 Function and Localization

• MRPL12 encodes the mitochondrial 39S ribosomal protein L12 (bL12m), a component of the large subunit of the mitochondrial ribosome.
• As part of the ribosome, MRPL12 contributes to mitochondrial protein synthesis, essential for oxidative phosphorylation. Related references: PMID:23603806
• In its non-ribosomal form, MRPL12 binds to mitochondrial RNA polymerase (POLRMT) to stimulate transcription, acting as a transcriptional activator. Related references: PMID:22003127
• MRPL12 is required for the stability of POLRMT, thereby linking ribosome biogenesis and mitochondrial gene expression. Related references: PMID:26586915
• The protein localizes to the mitochondrial matrix, consistent with its roles in ribosome and transcription complexes.
• Post-translational modifications include acetylation and ubiquitination, as identified by proteomics studies, and variants in MRPL12 are associated with primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length MRPL12 (aa 1-198), which may facilitate recognition of both ribosome-associated and free protein pools.
• For Western blotting, expect a band near 21 kDa; consider using mitochondrial-enriched lysates to enhance signal if expression is low.
• In immunofluorescence (IF/ICC), mitochondrial staining patterns should be validated with organelle markers.
• The antibody is reported to cross-react with mouse and rat; however, species-specific sequence homology should be verified for non-human applications.
• Preliminary titration is recommended for each application and sample type to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and researchers from concept to clinic with integrated raw-material sourcing, technical services, and consultation. For mitochondrial research targets like MRPL12, we offer ready-to-screen antibodies, validated antibody pairs, and bulk ancillary reagents to accelerate your diagnostic assay development. Our sourcing network can also address rare targets and custom requirements, ensuring a seamless supply chain.