Anti-MRPL12 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P52815

Core Product Specifications and Parameters

Biological Background: MRPL12 Function and Localization

• MRPL12, also designated as 5c5-2, L12mt, MRPL7, RPML12, MRPL7/L12, or MRP-L31/34, is the human mitochondrial large ribosomal subunit protein bL12m.
• As an integral ribosomal component, MRPL12 facilitates mitochondrial protein translation. Related references: PMID:23603806
• In its free, non-ribosome-bound form, MRPL12 binds to mitochondrial RNA polymerase POLRMT, thereby activating mitochondrial transcription. Related references: PMID:22003127
• MRPL12 is critical for maintaining POLRMT stability, underscoring its role in mitochondrial gene expression. Related references: PMID:26586915
• This protein resides exclusively in the mitochondrial matrix.
• Post-translational modifications of MRPL12 include acetylation and ubiquitination; it also possesses a transit peptide for mitochondrial import.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 46–198 of human MRPL12, a region that is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot analysis, a specific band at approximately 21 kDa is expected; consider using mitochondrial-enriched lysates for enhanced detection.
• In immunocytochemistry and immunohistochemistry, confirm mitochondrial localization by co-staining with a mitochondrial marker.
• When applying the antibody in ELISA, titrate optimally against the recombinant immunogen or native protein from relevant cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and research laboratories with a seamless supply of high-quality IVD raw materials. For studies on mitochondrial function and ribosomal biology, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies like this MRPL12 clone, and bulk ancillary reagents. Our team also assists with sourcing rare-target raw materials to support your assay development from concept to clinic.