Anti-MRP4/ABCC4 Rabbit Polyclonal Antibody for WB, ELISA - O15439

Core Product Specifications and Parameters

Biological Background: ATP-binding cassette sub-family C member 4 (ABCC4/MRP4) Function and Localization

• ATP-dependent transporter that actively extrudes physiological compounds and xenobiotics from cells, belonging to the ATP-binding cassette (ABC) family. Related references: PMID:11856762, PMID:12523936, PMID:15364914
• Transports endogenous signaling molecules such as cyclic AMP (cAMP), cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins, highlighting a key role in cellular communication. Related references: PMID:11856762, PMID:12523936, PMID:12835412
• Mediates the ATP-dependent efflux of glutathione conjugates like leukotriene C4 (LTC4) and leukotriene B4 (LTB4); GSH is required for LTB4 transport but not for LTC4. Related references: PMID:17959747
• Cotransports bile acids with reduced glutathione (GSH), expanding its role in hepatic and systemic homeostasis. Related references: PMID:12523936, PMID:12883481, PMID:16282361
• Transports a wide range of drugs and their metabolites, including anticancer, antiviral, and antibiotic agents, and confers resistance to methotrexate. Related references: PMID:11856762, PMID:12105214, PMID:15454390, PMID:11106685
• Localizes to both the basolateral and apical cell membrane, enabling directional transport in polarized cells.
• Widely expressed with particularly high levels in prostate; barely detectable in liver, except the sinusoidal membrane of hepatocytes.
• Keywords indicate additional features: alternative splicing, glycoprotein, phosphoprotein, and involvement in lipid transport and disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 1067-1325); the antibody likely recognizes full-length MRP4. Validate in the relevant sample system.
• For Western blot, consider using prostate-derived cell lines or MRP4-overexpressing models; expect a band around 150 kDa.
• For ELISA, optimize coating antigen and antibody dilutions; use recombinant MRP4 or cell lysates as positive controls.
• Cross-reactivity has only been tested against human; if studying other species, verify sequence homology of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether exploring multidrug resistance mechanisms or developing transport-related assays, our team can source rare-target raw materials like MRP4/ABCC4 antibodies to support your entire workflow from concept to clinic.