Anti-MRP4/ABCC4 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - O15439

Core Product Specifications and Parameters

Biological Background: MRP4/ABCC4 Function and Localization

• MRP4/ABCC4 is an ATP-binding cassette transporter that actively pumps a variety of physiological substrates, including cyclic nucleotides (cAMP, cGMP), bile acids, and steroid conjugates, out of cells (PubMed:11856762, PubMed:12523936, PubMed:15364914). Related references: PMID:11856762, PMID:12523936, PMID:15364914
• It mediates efflux of glutathione conjugates like leukotriene C4 and leukotriene B4; GSH is required for LTB4 but not LTC4 transport (PubMed:17959747). Related references: PMID:17959747
• Transports a wide array of drugs and their metabolites, including anticancer (e.g., methotrexate), antiviral, and antibiotic compounds, thereby contributing to multidrug resistance (PubMed:11856762, PubMed:12105214, PubMed:15454390, PubMed:11106685). Related references: PMID:11856762, PMID:12105214, PMID:15454390, PMID:11106685
• Localizes to both basolateral and apical plasma membranes; expressed widely with highest levels in prostate and barely detectable in liver, except on the sinusoidal membrane of hepatocytes.
• Post-translational modifications include glycosylation and phosphorylation; the protein has multiple transmembrane domains and contains nucleotide-binding domains.
• The gene ABCC4 encodes for MRP4, with alternative splicing producing different isoforms; aliases include MOATB, MOAT-B, and C4.
• Associated with disease phenotypes through genetic variants.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fragment covering amino acids 1067–1325; it may detect full-length MRP4 and certain isoforms. For Western blotting, expect a band around 150 kDa; consider using low‑percentage gels for high‑molecular‑weight separation.
• In immunohistochemical and immunofluorescence applications, appropriate fixation and antigen retrieval protocols should be validated for the tissue of interest.
• ELISA performance should be confirmed by checkerboard titration; the antibody can be used as a capture or detection reagent after pairing validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This Anti‑MRP4/ABCC4 monoclonal antibody is relevant for studies on drug transport, chemoresistance, and transporter biology. CamelBio can support your program with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your assay development from concept to clinic.