Anti-MRP4/ABCC4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15439

Core Product Specifications and Parameters

Biological Background: MRP4/ABCC4 Function and Localization

• MRP4 (ABCC4) is a member of the ATP-binding cassette (ABC) transporter superfamily, subfamily C, functioning as an ATP-dependent efflux pump for a wide variety of substrates. Related references: PMID:11856762, PMID:12523936, PMID:15364914
• It transports endogenous signaling molecules, including cyclic nucleotides (cAMP, cGMP), bile acids, steroid conjugates, urate, and prostaglandins. Related references: PMID:11856762, PMID:12523936, PMID:12835412
• The protein mediates efflux of glutathione conjugates like leukotriene C4 (LTC4) and leukotriene B4 (LTB4), with GSH required for LTB4 but not LTC4 transport. Related references: PMID:17959747
• MRP4 facilitates the cotransport of bile acids with reduced glutathione (GSH). Related references: PMID:12523936, PMID:12883481, PMID:16282361
• It handles a wide range of drugs and their metabolites, including anticancer, antiviral, and antibiotic agents, contributing to drug resistance. Related references: PMID:11856762, PMID:12105214, PMID:15454390
• MRP4 confers resistance to anticancer agents such as methotrexate. Related references: PMID:11106685
• Subcellular localization includes both basolateral and apical cell membranes, depending on tissue context.
• Tissue specificity: widely expressed, with particularly high levels in prostate, barely detectable in liver, and present at the sinusoidal membrane of hepatocytes.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1067–1325, which includes part of the nucleotide-binding domain 2 and the C-terminal region; it may recognize full-length MRP4 in denaturing conditions.
• For Western blot, the predicted molecular weight is ~150 kDa; consider using a 6–8% SDS-PAGE gel for optimal resolution.
• In IHC-P, antigen retrieval with citrate buffer (pH 6.0) or EDTA (pH 9.0) might be necessary; validate with appropriate positive and negative tissue controls.
• For IF/ICC, fixation with 4% PFA and permeabilization with 0.1% Triton X-100 is typically used; test antibody dilutions starting at 1:50–1:200.
• ELISA can be employed for quantitative detection; optimize coating antigen and antibody concentration according to your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to a comprehensive range of IVD raw materials, including validated polyclonal and monoclonal antibodies, matched antibody pairs, and ancillary reagents. For projects focused on drug resistance and ABC transporters like ABCC4, CamelBio supports your workflow with bulk ordering, custom antibody development, and technical consultation. Our sourcing capabilities help streamline your research from discovery to diagnostic application.