Anti-MRP3 Rabbit Polyclonal Antibody for IF-P, ELISA - O15438

Core Product Specifications and Parameters

Biological Background: MRP3 Function and Localization

• MRP3, encoded by ABCC3, is an ATP-binding cassette (ABC) transporter that uses ATP hydrolysis to actively transport diverse substrates including glucuronide conjugates, glutathione conjugates, and bile salts across cell membranes. Related references: PMID:10359813, PMID:11581266, PMID:15083066
• It mediates efflux of anticancer drugs such as methotrexate, teniposide, and etoposide, contributing to multidrug resistance in cancer cells. Related references: PMID:10359813, PMID:11581266
• MRP3 localizes to the basolateral membrane of hepatocytes and other epithelial cells, facilitating export of compounds from cells into the blood.
• Highly expressed in liver, with lower expression in small intestine, colon, prostate, testis, brain, and kidney, indicating tissue-specific transport roles. Related references: PMID:35307651
• In testis, MRP3 is found in peritubular myoid cells, Leydig cells, and along the basal membrane of Sertoli cells, suggesting a role in the blood-testis barrier. Related references: PMID:35307651
• The protein contains two nucleotide-binding domains and two transmembrane domains with multiple helices, characteristic of ABC transporters, and undergoes post-translational modifications such as phosphorylation.
• Alternative splicing generates multiple isoforms; the full-length protein has a molecular weight of approximately 169 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the intracellular region of MRP3; for applications beyond validated IF-P and ELISA, such as Western blot, consider in-house validation using whole-cell lysates or membrane preparations to confirm detection of the 169 kDa band.
• In ELISA, optimize coating antigen selection to match the immunogenic peptide region for improved sensitivity.
• For immunofluorescence (IF-P), effective permeabilization is necessary; test various fixation and permeabilization protocols with your specific cell model to ensure antibody access to the intracellular target.
• Given basolateral membrane localization in polarized cells, co-stain with basolateral markers to verify specificity.
• Reported cross-reactivity is human only; for other species, perform independent validation.
• To investigate phosphorylation or other modifications of MRP3, combine with targeted detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, including validated polyclonal and monoclonal antibodies for transporters like MRP3, along with bulk reagents, technical support, and rare-target sourcing. Whether advancing liver function diagnostics or oncology drug-resistance panels, CamelBio streamlines your path from concept to clinic with tailored sourcing and expert consultation.