Anti-MRP1/ABCC1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P33527

Core Product Specifications and Parameters

Biological Background: MRP1/ABCC1 Function and Localization

• MRP1/ABCC1 (Multidrug resistance-associated protein 1, also known as ATP-binding cassette sub-family C member 1, Leukotriene C4 transporter) is a member of the ABC transporter family, functioning as an ATP-dependent efflux pump for organic anions, drugs, and xenobiotics.
  Related references: PMID:10064732, PMID:11114332, PMID:16230346

• Plays a key role in multidrug resistance by exporting anticancer agents such as methotrexate and reducing their intracellular accumulation, with ATP hydrolysis being relatively inefficient.
  Related references: PMID:9281595, PMID:16230346

• Transports a broad spectrum of substrates including glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, and antiviral drugs, dependent on ATP and often glutathione.
  Related references: PMID:10064732, PMID:11114332

• Contributes to inflammatory responses by facilitating leukotriene C4 export from synthesizing cells and exports sphingosine 1-phosphate from mast cells independently of degranulation.
  Related references: PMID:17050692

• Regulates innate immunity by mediating ATP-dependent, GSH-independent cGAMP export, thereby limiting intracellular cGAMP and negatively regulating the cGAS-STING pathway.
  Related references: PMID:36070769

• Exhibits basolateral localization in polarized epithelial cells, enabling directional transport of GSH-conjugated molecules like prostaglandin A2 diastereomers, and also transports S-geranylgeranyl-glutathione to establish lymphoid tissue gradients.
  Related references: PMID:9426231

• Highly expressed in lung, testis, and peripheral blood mononuclear cells.

• Contains two nucleotide-binding domains and multiple transmembrane helices, consistent with ATP-binding cassette transporter architecture; post-translational modifications include phosphorylation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 850-960) maps to a cytoplasmic region, potentially offering accessibility in non-denaturing assays such as IF/ICC; validate permeabilization conditions for your cell type.
• For WB, expect bands around 172 kDa; however, post-translational modifications (glycosylation, phosphorylation) may cause slower migration—consider using 6–8% SDS-PAGE gels and appropriate molecular weight markers.
• Cross-reactivity with mouse and rat is reported; confirm antibody specificity in your model system using knockout controls or siRNA knockdown.
• For IHC-P, standard antigen retrieval (e.g., citrate buffer, pH 6.0) is recommended; optimize primary antibody concentration (start at 1:100–1:500) and detection method.
• In functional studies, note that MRP1/ABCC1 activity can be modulated by inhibitors such as MK-571; include appropriate controls to distinguish specific staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with reliable access to critical IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-MRP1/ABCC1 antibody for multidrug resistance and transport studies. Beyond antibodies, we offer bulk ancillary reagents and assist in sourcing rare targets to streamline your assay development from concept to clinic.