Anti-MRP1/ABCC1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P33527

Core Product Specifications and Parameters

Biological Background: MRP1/ABCC1 Function and Localization

• MRP1/ABCC1 (also designated ABCC1, MRP, GS-X) is a member of the ATP-binding cassette (ABC) transporter superfamily, encoded by the ABCC1 gene. Alternative splicing produces multiple isoforms.
• Core efflux function: Mediates ATP-dependent export of a broad spectrum of organic anions, including glutathione conjugates, leukotriene C4, and anticancer drugs, thereby conferring multidrug resistance. Hydrolyzes ATP with low efficiency.
  Related references: PMID:10064732, PMID:11114332, PMID:16230346, PMID:9281595
• Sphingolipid transport: Exports sphingosine-1-phosphate (S1P) from mast cells independently of degranulation, influencing immune cell trafficking.
  Related references: PMID:17050692
• Innate immune regulation: Transports cyclic GMP-AMP (cGAMP) to limit intracellular levels and negatively regulate the cGAS-STING pathway, modulating antiviral responses.
  Related references: PMID:36070769
• Subcellular localization: Predominantly localized to the basolateral membrane in polarized epithelial cells, consistent with its role in xenobiotic and metabolite efflux.
• Tissue distribution: Highly expressed in lung, testis, and peripheral blood mononuclear cells (PBMCs), reflecting its physiological roles in barrier tissues and immune cells.
• Structural domains: Possesses two nucleotide-binding domains (NBDs) and two transmembrane domains (TMDs), typical of full-length ABC transporters. Post-translational modifications include N-linked glycosylation.

Experimental Guidance and Technical Tips

• Western Blotting: The predicted molecular weight is 172 kDa; use a low-percentage gel (e.g., 6–8%) and appropriate molecular weight markers. Reducing conditions are recommended.
• IHC-P: For paraffin-embedded tissue sections, antigen retrieval may be necessary. Test with known positive tissues such as human lung or testis. Optimize antibody dilution for each sample type.
• ELISA: Suitable for detection of MRP1 in cell lysates or recombinant protein. Validate assay conditions by checkerboard titration.
• Species Cross-reactivity: The antibody is reported to cross-react with mouse and rat MRP1, but sequence alignment of the immunogen region suggests potential reactivity; confirm with appropriate controls.
• Immunogen Note: The immunogen spans aa 210–304 within a cytosolic loop, which is highly conserved across species, supporting multi-species utility.

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