Anti-MRI1 Polyclonal Antibody for WB & ELISA - Q9BV20

Core Product Specifications and Parameters

Biological Background: MRI1 Function and Localization

• MRI1 catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) and methylthioribulose-1-phosphate (MTRu-1-P), a key step in the methionine salvage pathway.
• Beyond its isomerase activity, MRI1 functions as a mediator of RhoA-dependent cancer cell invasion by enhancing FAK tyrosine phosphorylation and stress fiber turnover.
• The protein localizes to the nucleus, cytoplasm, and cell projections, suggesting context-dependent roles in metabolism and cell motility.
• Alternative names include Mediator of RhoA-dependent invasion (MRDI), S-methyl-5-thioribose-1-phosphate isomerase, and Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein.
• Post-translational modifications such as acetylation and alternative splicing have been documented for MRI1.
• UniProt keywords (Isomerase, Methionine biosynthesis, Cell projection) highlight its dual roles in amino acid metabolism and cellular dynamics.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–369 of human MRI1) covers the full-length protein; therefore, this polyclonal antibody may detect both native and denatured forms in WB.
• For ELISA, consider using a recombinant MRI1 protein as a positive control to establish assay sensitivity.
• Cross-reactivity with mouse and rat is indicated; for cross-species applications, validate specificity in the relevant tissue or cell line.
• The expected molecular weight of MRI1 is approximately 39 kDa (UniProt: 39,150 Da); in WB, a band near this size should be observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive, one-stop sourcing solution for IVD raw materials. This anti-MRI1 polyclonal antibody supports investigations into methionine metabolism and cancer cell invasion pathways. Beyond individual antibodies, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized procurement of rare-target raw materials to streamline your assay development.