Anti-MRFAP1 Polyclonal Antibody for WB and ELISA - Q9Y605

Core Product Specifications and Parameters

Biological Background: MRFAP1 Function and Localization

• MRFAP1 (MORF4 family-associated protein 1) is a 14.6 kDa protein that harbors a coiled-coil domain, suggesting a role in mediating protein-protein interactions.
• Also known as PGR1 and PAM14 (Protein Associated with MRG of 14 kDa), it associates with the MORF4-related gene (MRG) family, which is implicated in cellular senescence and transcription regulation.
• Subcellular fractionation and immunofluorescence reveal localization to the nucleus, cytoplasm, and perinuclear region, indicating dynamic shuttling or multiple functional compartments.
• The protein is a member of the reference proteome and has been identified through large-scale proteomics studies, but detailed functional characterization remains limited.
• Coiled-coil motifs in MRFAP1 likely facilitate homodimerization or heterodimerization with MRG proteins, contributing to chromatin-modifying complexes.

Experimental Guidance and Technical Tips

• For Western blot, the expected band size is approximately 15 kDa; include a whole-cell lysate from human cell lines (e.g., HeLa, HEK293) and validate with a positive control such as the recombinant immunogen.
• When using ELISA, consider coating the plate with the recombinant protein immunogen or a specific peptide to assess antibody titer and specificity.
• As a polyclonal antibody, lot-to-lot consistency should be verified by performing a side-by-side comparison with a previously validated lot in your assay system.
• Optimization of antibody dilution and incubation conditions is recommended, as performance may vary depending on sample preparation and detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. For MRFAP1-related research and assay development, we supply validated rabbit polyclonal antibodies and can facilitate procurement of antibody pairs, bulk ancillary reagents, and rare-target raw materials. Our integrated services streamline supply chains, enabling rapid translation of transcriptional regulation discoveries into diagnostic applications.