Anti-MRE11 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P49959

Core Product Specifications and Parameters

Biological Background: MRE11 Function and Localization

• MRE11 (Meiotic recombination 11 homolog 1) is a core component of the MRN complex (MRE11-RAD50-NBN), essential for DNA double-strand break repair, homologous recombination, telomere maintenance, and meiosis. Related references: PMID:11741547, PMID:14657032, PMID:22078559
• The MRN complex initiates end resection at DSBs: MRE11 endonucleolytically cleaves the 5′ strand and then performs 3′-5′ exonucleolytic degradation, creating a 3′ overhang for long-range resection by EXO1/DNA2. Related references: PMID:24316220, PMID:28867292
• The endonuclease activity of MRE11 is stimulated by RBBP8/CtIP to clear protein-DNA adducts, and by AGER/RAGE (by similarity); this activity licenses homologous recombination by preventing non-homologous end joining. Related references: PMID:27814491, PMID:27889449, PMID:30787182
• MRE11 also has a structural role in ATM/ATR kinase activation (independent of its nuclease activity) and in processing R-loops. Related references: PMID:14657032, PMID:15064416, PMID:15790808, PMID:31537797
• The MRN complex is involved in activating the cGAS-STING pathway during tumorigenesis by displacing CGAS from nucleosomes (by similarity).
• Localizes to the nucleus, chromosomes, and telomeres; modulates t-loop formation at telomeres. Related references: PMID:10888888
• Post-translational modifications include phosphorylation, acetylation, methylation, and ubiquitination, which regulate its activity and interactions.
• Alternatively spliced isoforms exist; mutations in MRE11 cause ataxia-telangiectasia-like disorder (ATLD) and are associated with ciliopathies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-302) of human MRE11. This region contains DNA-binding domains and may be suitable for detecting full-length and N‑terminal isoforms.
• For Western blot (WB), a band at ~81 kDa is expected. Use appropriate loading controls and consider the expression profile of MRE11 across human and mouse tissue or cell lines.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody dilution may need optimization; nuclear and chromosomal staining is anticipated.
• For ELISA, the recombinant immunogen or native protein can be used as coating antigen; cross-reactivity with human and mouse MRE11 has been demonstrated.
• The antibody has not been tested in other applications; users should validate performance in their specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. This MRE11 antibody supports research in DNA damage repair, oncology, and telomere biology, and CamelBio can assist with scaling up for diagnostic development and sourcing rare-target materials.