Anti-MRE11 Rabbit Monoclonal Antibody for WB, ELISA, ChIP - P49959

Core Product Specifications and Parameters

Biological Background: MRE11 Function and Localization

• MRE11 is a core component of the MRN complex, playing a central role in DNA double-strand break (DSB) repair, homologous recombination (HR), telomere maintenance, and meiosis.
  Related references: PMID:11741547, PMID:14657032, PMID:22078559
• MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are essential for initiating end resection at DSBs.
  Related references: PMID:11741547, PMID:22078559, PMID:24316220
• Following DNA damage, MRE11 cooperates with RBBP8/CtIP to cleave the 5' strand endonucleolytically, then generates a single-stranded DNA gap via exonucleolytic degradation, facilitating long-range resection by EXO1 and DNA2.
  Related references: PMID:24316220, PMID:27814491, PMID:27889449, PMID:28867292
• The nuclease activity of MRE11 is dispensable for ATM and ATR kinase activation, highlighting distinct structural roles in DNA damage signaling.
  Related references: PMID:14657032, PMID:15064416, PMID:15790808
• MRE11 localizes to the nucleus, chromosomes, and telomeres, where it modulates t-loop formation and telomere integrity.
• MRE11 contains two DNA-binding domains, enabling it to bind both single-stranded and double-stranded DNA.
• Post-translational modifications of MRE11 include acetylation, methylation, phosphorylation, and ubiquitination. Alternative splicing generates multiple isoforms.
• Mutations in MRE11 are associated with ataxia-telangiectasia-like disorder (ATLD) and ciliopathies.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide mapping to amino acids 400–500 of human MRE11 (P49959). For applications such as ChIP, consider optimizing fixation and antibody titration.
• This rabbit monoclonal antibody is reactive with human and mouse MRE11; cross-reactivity with other species should be verified independently.
• In Western blotting, a band corresponding to approximately 81 kDa is expected. Validate the observed molecular weight against appropriate controls in your sample system.
• For ELISA and ChIP applications, pair with validated secondary antibodies and perform preliminary tests to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials. For DNA repair research and assay development targeting MRE11 and the MRN complex, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. From lead identification to scale-up, CamelBio bridges your entire development pipeline.