Anti-MRE11 Polyclonal Antibody for WB, IP, ELISA - P49959

Core Product Specifications and Parameters

Biological Background: MRE11 Function and Localization

• MRE11 is a core component of the MRN complex, essential for double-strand break repair, DNA recombination, telomere maintenance, and meiosis. Related references: PMID:11741547, PMID:14657032, PMID:22078559
• The MRN complex facilitates homologous recombination repair by mediating DNA end resection. MRE11 first endonucleolytically cleaves the 5' strand to prevent NHEJ, then generates 3' single-stranded DNA via exonuclease activity, enabling long-range resection by EXO1 and DNA2. Related references: PMID:24316220, PMID:28867292
• MRE11 possesses both single-strand endonuclease and double-strand-specific 3'-5' exonuclease activities. Its endonuclease activity is specifically promoted by RBBP8/CtIP to clear protein-DNA adducts and generate clean DSB ends. Related references: PMID:11741547, PMID:22078559, PMID:24316220, PMID:27814491, PMID:27889449, PMID:30787182
• The MRN complex is required for DNA damage signaling through ATM and ATR kinase activation, a role independent of MRE11 nuclease activity. Related references: PMID:14657032, PMID:15064416, PMID:15790808
• In telomeres, the MRN complex may modulate t-loop formation, contributing to telomere integrity. Related references: PMID:10888888
• The MRN complex is also involved in R-loop processing and activation of the cGAS-STING innate immune pathway during tumorigenesis. Related references: PMID:31537797
• MRE11 contains two DNA-binding domains, enabling binding to both single-stranded and double-stranded DNA.
• MRE11 is subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and ubiquitination, and mutations are linked to disease variants and ciliopathy.
• MRE11 localizes to the nucleus, chromosomes, and telomeres.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 290-485 of human MRE11, a central region. This polyclonal antibody is expected to detect full-length MRE11 (81 kDa) in denaturing western blot (WB). Optimize the primary antibody dilution (e.g., 1:500-1:2000) for your experimental system.
• For immunoprecipitation (IP), consider using at least 2-5 µg of antibody per 500 µg of cell lysate. Include appropriate positive and negative controls, and validate the target band by mass spectrometry or siRNA knockdown if needed.
• In ELISA applications, titrate the antibody against the immunogen or purified protein to determine the optimal working concentration. Confirm specificity by testing against unrelated antigens.
• Since cross-reactivity is reported for human and rat, validate the antibody in your species and tissue of interest using positive controls known to express MRE11.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop supplier of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. For DNA repair and MRE11-focused research projects, we provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. Whether you need assay-matched reagents or custom procurement solutions, CamelBio bridges your MRE11 research needs with reliable supply capabilities.