Anti-MRC2 Polyclonal Antibody for WB, ELISA - Q9UBG0

Core Product Specifications and Parameters

Biological Background: MRC2 Function and Localization

• MRC2, also known as CD280, ENDO180, UPARAP, or CLEC13E, is a type I transmembrane C-type lectin receptor (Gene ID: 9902) involved in endocytosis and extracellular matrix remodeling.
• It functions as a calcium-dependent endocytotic lectin receptor, internalizing glycosylated ligands via clathrin-mediated endocytosis and directing them to endosomal compartments.
• MRC2 may contribute to the regulation of the plasminogen activation system by controlling extracellular levels of PLAUR/PLAU, thereby modulating cell-surface protease activity.
• The receptor plays a key role in collagen turnover by mediating the uptake, remodeling, and degradation of extracellular collagen matrices, often in cooperation with matrix metalloproteinases (MMPs). This function implicates MRC2 in cancer progression and chronic tissue-destructive diseases.
• MRC2 is ubiquitously expressed at low levels in most adult tissues, including brain, placenta, lung, kidney, pancreas, and colon, with higher expression in fetal lung and kidney. It is predominantly found in endothelial cells, fibroblasts, and macrophages.
• As a membrane glycoprotein, MRC2 contains structural features such as a signal peptide, a cysteine-rich domain, a fibronectin type II domain, multiple C-type lectin domains, a transmembrane helix, and a short cytoplasmic tail. It undergoes post-translational modifications including N-glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 120-300 of human MRC2) maps to the extracellular region, suggesting the antibody recognizes the receptor's extracellular domain. For Western blot, consider a starting dilution range of 1:500–1:2000 under reducing conditions, and expect a band near 167 kDa. Validate using positive control lysates from cell lines such as HeLa, human fibroblasts, or macrophages.
• For ELISA applications, use the recombinant immunogen as a standard and optimize coating and detection conditions empirically. The antibody's cross-reactivity with mouse MRC2 enables its use in mouse model studies; however, users should independently verify reactivity in their specific mouse strain or tissue.
• Due to the polyclonal nature, this antibody may exhibit lot-to-lot variability. It is advisable to reserve a retained sample for bridging studies, and to validate performance in the intended assay system before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of antibodies, antigens, and ancillary reagents to diagnostic manufacturers and research laboratories. For projects involving collagen remodeling, cancer biology, or endocytosis pathways, we supply this anti-MRC2 polyclonal antibody along with other validated reagents such as MMP antibodies, recombinant collagens, and ELISA-matched pairs. Our team can assist with custom antibody development, bulk orders, and sourcing rare targets, helping you accelerate assay development from concept to clinic.