Anti-MPZL1 Polyclonal Antibody for WB, ELISA - O95297

Core Product Specifications and Parameters

Biological Background: MPZL1 Function and Localization

• MPZL1 (Myelin protein zero-like protein 1), also known as PZR, is a cell surface receptor involved in signal transduction processes.
• It recruits the tyrosine phosphatase PTPN11/SHP-2 to the cell membrane and is a putative substrate of SHP-2.
• MPZL1 serves as a major receptor for concanavalin-A (ConA), mediating ConA-induced cellular signaling that probably involves Src family tyrosine-protein kinases.
• Isoform 3 has a dominant negative role, blocking ConA-induced tyrosine phosphorylation of MPZL1.
• Isoform 1, but not isoforms 2 and 3, may be involved in regulation of integrin-mediated cell motility.
• The protein localizes to the membrane and contains a signal sequence, an immunoglobulin domain, a transmembrane helix, and a cytoplasmic tail.
• It is widely expressed, with highest levels in heart, placenta, kidney, and pancreas; isoform 1 is prominent in stromal cells and isoform 3 in hematopoietic tissues.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 195–269 in the intracellular region of human MPZL1; verify reactivity with relevant isoforms, as isoform expression can vary by cell type.
• For Western blot, a band near the predicted molecular weight of ~29 kDa is expected; glycosylation may cause a slight shift.
• The antibody is suitable for ELISA; consider optimizing coating conditions and pairing with a detection antibody for sandwich ELISA development.
• This polyclonal antibody recognizes human, mouse, and rat MPZL1; validate in your specific sample matrix to confirm cross-reactivity.
• As MPZL1 is involved in ConA signaling and integrin-mediated cell motility, consider appropriate stimulation or functional assays to confirm antibody specificity in context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with tailored access to IVD raw materials and services, supporting projects from concept to clinic. For researchers investigating immune receptor signaling or cell adhesion, we can supply validated anti-MPZL1 antibodies, matched antibody pairs for immunoassays, and bulk ancillary reagents. Our sourcing network also facilitates access to rare-target raw materials, ensuring a reliable supply chain for your assay development needs.