Anti-MPP1 Polyclonal Antibody for WB, ELISA - Q00013

Core Product Specifications and Parameters

Biological Background: MPP1 Function and Localization

• MPP1, also known as 55 kDa erythrocyte membrane protein or membrane protein palmitoylated 1, is encoded by the MPP1 gene (also referred to as DXS552E or EMP55) and belongs to the membrane-associated guanylate kinase (MAGUK) family.
• It acts as an essential regulator of neutrophil polarity by modulating AKT1 phosphorylation through a PIK3CG-independent mechanism (by similarity).
• MPP1 is a multidomain scaffolding protein containing an SH3 domain, which mediates protein-protein interactions critical for its function.
• It localizes to the cell membrane and is enriched in stereocilia, specialized actin-based cell projections involved in mechanosensation.
• The protein is ubiquitously expressed across tissues, reflecting its fundamental role in cellular organization and signaling.
• Post-translational modifications include palmitoylation, which anchors MPP1 to the membrane, and phosphorylation, which may regulate its activity and interactions.
• Alternative splicing generates multiple isoforms, contributing to functional diversity (as indicated by proteomics identification and reference proteome annotations).
• Acetylation has been detected, suggesting additional regulatory layers.

Experimental Guidance and Technical Tips

• This antibody is recommended for Western blot (WB) and ELISA applications; ensure appropriate dilution and detection conditions are optimized.
• The immunogen covers the N-terminal region (aa 1-270) of human MPP1; cross-reactivity with mouse has been confirmed, and reactivity with other species may require verification.
• Predicted molecular weight is 52 kDa; in WB, consider using a suitable protein ladder and confirm band size.
• As a polyclonal antibody, it may recognize multiple isoforms or post-translationally modified forms; validate specificity in the relevant biological context.
• For ELISA, pair with a suitable detection antibody and use appropriate antigen coating conditions.
• Store according to shipping conditions (ice bag) and avoid repeated freeze-thaw cycles to maintain activity.

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