Anti-MPG Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - P29372

Core Product Specifications and Parameters

Biological Background: MPG Function and Localization

• MPG (N-methylpurine-DNA glycosylase, also known as AAG, ANPG, ADPG) is a monofunctional DNA glycosylase that initiates base excision repair by catalyzing hydrolysis of the N-glycosidic bond to release damaged bases.
• It specifically recognizes and excises 3-methyladenine and 7-methylguanine from DNA alkylated by endogenous and exogenous agents, preventing mutations and genotoxicity.
• The enzyme localizes to both the nucleus and mitochondria (including mitochondrial nucleoids), reflecting its role in maintaining genomic and mitochondrial DNA integrity.
• Alternative splicing generates multiple isoforms, with the full-length protein (293 amino acids) weighing approximately 33 kDa.
• MPG is classified as a phosphoprotein and belongs to the DNA damage repair pathway, highlighted by UniProt keywords: DNA damage, DNA repair, Hydrolase, Reference proteome.
• Functional studies indicate its critical role in protecting cells against alkylating chemotherapeutics and reactive oxygen species-induced lesions.

Experimental Guidance and Technical Tips

• The immunogen used is a full-length recombinant human MPG protein (amino acids 1–293), so the antibody likely recognizes native and denatured MPG across multiple species due to high sequence homology.
• For Western blot, consider testing a range of dilutions starting from 1:1000; the expected band is around 33 kDa. Include KO lysate as a negative control to confirm specificity.
• For ELISA, the polyclonal nature may provide strong signal; titrate the antibody and validate with recombinant MPG or cell lysates.
• Verify expression in your model system; MPG is ubiquitously expressed but may be upregulated under DNA-damaging conditions.

CamelBio: Your One-Stop Sourcing Bridge

Targeting the DNA repair field, CamelBio supplies validated IVD raw materials including polyclonal antibodies, recombinant proteins, and assay-matched reagent sets. Our portfolio covers key enzymes like MPG for genotoxicity and cancer research, supporting your development from early-stage assay design to scalable manufacturing. As a one-stop sourcing partner, we also assist with rare target antibody generation and bulk ancillary reagent procurement to streamline your diagnostic workflow.