Anti-MPDZ Rabbit Polyclonal Antibody for WB, ELISA - O75970

Core Product Specifications and Parameters

Biological Background: MPDZ Function and Localization

• MPDZ (Multiple PDZ domain protein) is also known as MUPP1 (Multi-PDZ domain protein 1). It belongs to a family of PDZ domain-containing scaffolding proteins.
• It functions as a member of the NMDAR signaling complex and plays a role in the control of AMPAR potentiation and synaptic plasticity in excitatory synapses. Related references: PMID:11150294, PMID:15312654
• Promotes clustering of the serotonin receptor HT2RC (5-HT2C) at the cell surface (By similarity).
• Subcellular localization includes cell membrane, apical cell membrane, postsynaptic density, dendrite, tight junctions, synapse, and synaptosome.
• Tissue expression is detected in heart, brain, placenta, liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include phosphorylation and methylation, and the gene undergoes alternative splicing to produce multiple isoforms.
• Contains multiple PDZ domains, enabling interaction with various proteins, including receptors and cytoskeletal elements, contributing to cell junction and synapse organization.
• Has been implicated in host-virus interactions (UniProt keyword).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to amino acids 1100-1380 of human MPDZ (NP_003820.2). This region may generate an antibody recognizing native MPDZ in various species, as suggested by the reported cross-reactivity with human, mouse, and rat.
• For Western blot (WB), note the predicted protein weight of ~222 kDa. Consider using a low-percentage acrylamide gel (e.g., 6-8%) and extended transfer times to ensure efficient transfer of high-molecular-weight proteins. Validate the band specificity in your sample system.
• For ELISA applications, the antibody may be used as a capture or detection reagent; however, optimal dilutions and conditions should be empirically determined using the relevant recombinant protein or cell lysates.
• Cross-reactivity with mouse and rat MPDZ is indicated; verify sequence homology in the immunogen region to assess potential reactivity in other species or with protein isoforms.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing partner, CamelBio provides diagnostic developers and researchers with reliable access to critical raw materials, from antibodies to ancillary reagents. This anti-MPDZ polyclonal antibody serves as a valuable tool for investigating synaptic plasticity, tight junction biology, and scaffold protein interactions. CamelBio's comprehensive supply capabilities also encompass validated antibody pairs, optimized monoclonal/polyclonal antibodies, and rare-target sourcing to support your entire IVD development pipeline.