Anti-MOV10 Rabbit Monoclonal Antibody for WB, ELISA - Q9HCE1

Core Product Specifications and Parameters

Biological Background: MOV10 Function and Localization

• MOV10 (Armitage homolog, fSAP113) is an RNA helicase with 5'-3' unwinding activity, belonging to the UPF1-like helicase family.
• It plays a central role in innate antiviral immunity by promoting type I interferon production through IKKepsilon/IKBKE-mediated IRF3 activation. Related references: PMID:27016603, PMID:27974568, PMID:35157734
• MOV10 restricts HIV-1 replication at a post-entry step and counteracts Vif-mediated degradation of APOBEC3G via its helicase activity. Related references: PMID:20215113, PMID:29258557
• It inhibits hepatitis B virus (HBV) replication by binding HBV RNA and blocking the early step of viral reverse transcription. Related references: PMID:31722967
• MOV10 is required for microRNA-mediated gene silencing, participating in both translational repression and target mRNA cleavage by the RISC complex. Related references: PMID:16289642, PMID:17507929, PMID:22791714
• It restricts LINE-1 retrotransposition by facilitating TUT4/TUT7-mediated uridylation of LINE-1 RNA. Related references: PMID:23093941, PMID:30122351
• The protein localizes to the cytoplasm, P-bodies, stress granules, and nucleus, reflecting its multifunctional roles in RNA metabolism.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• For Western blotting, expect a specific band near the calculated molecular weight of approximately 114 kDa; validate detection in your sample system using appropriate positive and negative controls.
• The immunogen corresponds to a peptide within amino acids 800–900, a region potentially conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• As a rabbit monoclonal antibody, it offers batch-to-batch consistency; consider optimizing antibody dilution and incubation conditions for your specific assay format.
• For ELISA, confirm coating antigen and detection conditions using recombinant MOV10 protein or cell lysates expressing the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research laboratories, providing one-stop access to high-quality IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare targets like MOV10. Whether you are developing assays for antiviral research, retrotransposon biology, or miRNA pathway studies, CamelBio streamlines your procurement process from concept to clinic.