Anti-Mouse/Rat CD90.1 Monoclonal Antibody for Flow Cytometry - P01831

Core Product Specifications and Parameters

Biological Background: Thy-1 (CD90.1) Function and Localization

• Encoded by the Thy1 gene, Thy-1 (CD90.1) is a GPI-anchored membrane glycoprotein of the immunoglobulin superfamily, characterized by a single immunoglobulin-like domain and multiple disulfide bonds.
• The protein is localized to the external surface of the cell membrane via a glycosylphosphatidylinositol (GPI) anchor, enriching it in lipid rafts and facilitating signal transduction.
• Functional studies suggest a role in cell-cell and cell-ligand interactions, particularly during synaptogenesis and other developmental events in the brain.
• Post-translational modifications include N-terminal pyrrolidone carboxylic acid formation, glycosylation, and disulfide bond formation, which are essential for its structural stability and biological activity.
• In mouse and rat, two allelic forms—Thy-1.1 (CD90.1) and Thy-1.2 (CD90.2)—are distinguished by sequence polymorphisms, providing a basis for allele-specific antibody recognition.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning the extracellular domain of mouse/rat CD90.1; this design may favor recognition of native conformational epitopes, making it well-suited for live-cell flow cytometry.
• For FC, titrate the antibody to determine the optimal concentration for your target cell population; staining intensity can vary between tissues and with cell activation states.
• As CD90.1 is a GPI-anchored surface protein, permeabilization is not required; surface staining can be combined with fixation if intracellular markers are co-stained.
• When using with other fluorophore-conjugated antibodies, perform appropriate single-stain and compensation controls to avoid spectral overlap artifacts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of raw materials, including this monoclonal antibody for CD90.1 (Thy-1). Our integrated supply platform supports IVD development from initial screening to scale-up, covering immunoassay reagents, ancillary buffers, and specialized technical consulting. Whether you need validated antibody pairs, bulk quantities, or rare target sourcing, CamelBio streamlines your procurement for immunophenotyping and other research applications.