Anti-Mouse/Rat CD31/PECAM1 Monoclonal Antibody () for Flow Cytometry - Q08481

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31/PECAM1 is an immunoglobulin superfamily cell adhesion molecule essential for leukocyte transendothelial migration (TEM). Tyr-679 is critical for trafficking to the lateral border recycling compartment (LBRC).
• It mediates homophilic endothelial cell-cell adhesion at cell junctions, contributing to vascular barrier integrity.
• Heterophilic binding to CD177 facilitates neutrophil transendothelial migration.
• Homophilic ligation of PECAM1 on viable leukocytes transmits a detachment signal that prevents macrophage-mediated phagocytosis, while on apoptotic cells this signal is disabled, promoting tethering and clearance.
• PECAM1 modulates bradykinin receptor BDKRB2 activation and regulates ERK1/2 signaling in endothelial cells.
• It has been implicated in atherosclerosis susceptibility in mice. Related references: PMID:19048083
• The protein localizes to cell membrane rafts and cell junctions, and undergoes post-translational modifications including palmitoylation, phosphorylation, and N-glycosylation.
• Expressed in lung vasculature and platelets.

Experimental Guidance and Technical Tips

• For flow cytometry, use live, unfixed cells to preserve surface CD31 expression; the immunogen (aa 18-220) spans the extracellular domain, suggesting the antibody binds an extracellular epitope.
• Titrate the antibody to determine the optimal staining concentration for your cell type of interest (e.g., mouse endothelial cells or platelets).
• Include appropriate isotype controls and single-stain compensation controls when performing multicolor flow cytometry.
• Validate cross-reactivity in rat samples if applicable, as the antibody is reported to recognize rat CD31.

CamelBio: Your One-Stop Sourcing Bridge

For researchers investigating endothelial biology, vascular inflammation, or atherosclerosis, CamelBio provides comprehensive access to CD31 antibodies and related reagents. As a one-stop IVD raw-material sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and facilitated rare-target raw-material procurement to support your diagnostic and research workflows.