Anti-Mouse Ythdc1 Polyclonal Antibody for WB - E9Q5K9

Core Product Specifications and Parameters

Biological Background: YTH domain-containing protein 1 Function and Localization

• Recognizes and binds N6-methyladenosine (m6A)-containing RNAs, acting as a key regulator of alternative splicing. It promotes exon inclusion by recruiting SRSF3 to m6A sites, or prevents exon skipping by interfering with SRSF10 binding (PubMed:29262316, PubMed:29799838). Related references: PMID:29262316 PMID:29799838
• Facilitates nuclear export of m6A-containing mRNAs through interactions with SRSF3 and NXF1.
• Controls S-adenosyl-L-methionine homeostasis by modulating the expression of MAT2A transcripts, likely via m6A binding within these mRNAs (PubMed:29262316). Related references: PMID:29262316
• Participates in random X chromosome inactivation by binding m6A-modified Xist RNA and promoting its transcription-repressive function.
• Essential for germline development, specifically spermatogonial development in males and oocyte growth/maturation in females, primarily through its alternative splicing regulatory role (PubMed:29799838). Related references: PMID:29799838
• Independently of its m6A reader activity, it stabilizes PPARG by preventing ARIH2-mediated ubiquitination, thereby promoting brown adipose tissue development and energy homeostasis (PubMed:40355558). Related references: PMID:40355558
• Localizes predominantly to the nucleus and nuclear speckles, consistent with its roles in mRNA processing.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation (isopeptide bond formation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 597-736 of mouse Ythdc1, a C-terminal region that may be accessible in native and denatured protein conformations. This region excludes the known YTH domain (approx. residues 400–540), potentially reducing cross-reactivity with other m6A reader proteins, though experimental validation is recommended.
• For Western blotting (WB), anticipate a band at approximately 85 kDa in mouse tissue lysates. Use reducing conditions and validate with appropriate positive and negative controls (e.g., Ythdc1 knockout tissues or cells) to confirm specificity.
• In ELISA applications, the recombinant protein used as immunogen can serve as a positive control standard to optimize coating concentrations and antibody dilutions.
• As this is a polyclonal antibody, it may recognize multiple epitopes, providing robust signal but also an increased risk of background. Include blocking agents and optimization steps suitable for polyclonal antibodies in your protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, CROs, and research laboratories by providing a comprehensive portfolio of IVD raw materials and expert technical services. This anti-Ythdc1 polyclonal antibody addresses the growing demand for reliable tools in epitranscriptomics and RNA modification research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare-target raw materials—all designed to streamline your assay development from concept to clinic.