Anti-Mouse Siglec5/Siglec-F Monoclonal Antibody () for FC - Q920G3

Core Product Specifications and Parameters

Biological Background: Siglec5/Siglec-F Function and Localization

• Siglec5 (also known as Siglec-F or mSiglec-F) is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family.
• It functions as a putative adhesion molecule that mediates sialic-acid dependent binding to cells, with a preference for alpha-2,3-linked sialic acid.
• The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
• Siglec5 is a transmembrane protein located on the cell membrane.
• It is predominantly expressed by immature monocytic/myeloid lineage cells in bone marrow, and at lower levels in mature neutrophils and monocytes.
• The protein contains immunoglobulin domains and is classified as a lectin; it features a signal peptide, disulfide bonds, and multiple repeats.
• Its molecular weight is approximately 61 kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 18-437) covers the extracellular domain, which includes the sialic acid-binding region, supporting surface expression detection.
• This conjugate is designed for flow cytometry (FC); perform antibody titration and include appropriate isotype controls for optimal staining.
• Validate specificity in mouse myeloid-lineage cells (e.g., bone marrow or peripheral blood) where Siglec5 expression is expected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting. This anti-mouse Siglec5 monoclonal antibody exemplifies our commitment to supplying high-quality tools for immune cell analysis. We support your entire workflow with validated antibody pairs, recombinant proteins, bulk reagents, and rare-target sourcing—from early-stage research to clinical assay development.