Anti-Mouse Podoplanin Monoclonal Antibody for FC - Q62011

Core Product Specifications and Parameters

Biological Background: Podoplanin Function and Localization

• Podoplanin is a type I transmembrane glycoprotein also known as Gp38, T1alpha, and OTS-8, characterized by a heavily O-glycosylated extracellular domain that mediates interactions with multiple binding partners.
• It plays a critical role in blood and lymphatic vessel separation during development by binding to CLEC1B on platelets, which triggers platelet activation and aggregation. Related references: PMID:14522983, PMID:15231832, PMID:17616532
• Interaction with CD9 attenuates platelet aggregation and pulmonary metastasis; through MSN or EZR, it promotes epithelial-mesenchymal transition (EMT), leading to RHOA activation and increased cell migration and invasiveness.
• In lymph nodes, podoplanin on fibroblastic reticular cells engages CLEC1B, regulating cell adhesion to the extracellular matrix and actomyosin contraction by modulating ERM proteins and MYL9 activation. Related references: PMID:25347465
• In keratinocytes, podoplanin expression induces elongated morphology, actin cytoskeleton reorganization, enhanced motility, and decreased cell adhesion. Related references: PMID:10574709
• It is essential for normal lung cell proliferation and alveolus formation at birth. Related references: PMID:12654292
• Subcellularly, podoplanin localizes to the membrane of lamellipodia, filopodia, microvilli, ruffles, membrane rafts, and invadopodia; it is expressed at high levels in lung and brain, with lower levels in kidney, stomach, liver, spleen, and esophagus.
• Although structurally related to aquaporins, podoplanin does not function as a water channel or affect folic acid/amino acid transport. Related references: PMID:12032185

Experimental Guidance and Technical Tips

• For flow cytometry, include appropriate isotype controls and consider Fc receptor blocking to reduce non-specific binding.
• Titrate the antibody to determine the optimal concentration for your cell type and experimental setup.
• Since the immunogen covers amino acids 21–133 of mouse podoplanin, the antibody likely recognizes an extracellular epitope suitable for live-cell staining; validate in known positive cell lines or tissues (e.g., lung epithelial or lymphatic endothelial cells).
• Maintain careful handling under ice-bag shipping conditions to preserve conjugate stability and activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. With expertise in rare targets such as podoplanin for cancer and developmental research, we support assay development from concept to clinic. Contact us to streamline your procurement and secure high-quality raw materials tailored to your diagnostic needs.