Anti-Mouse Placenta Growth Factor (PlGF) Polyclonal Antibody for WB and ELISA - P49764

Core Product Specifications and Parameters

Biological Background: Placenta Growth Factor Function and Localization

• Placenta growth factor (PlGF) is a secreted glycoprotein belonging to the vascular endothelial growth factor (VEGF) family, encoded by the Pgf gene (Gene ID: 18654) in mouse.
• It functions as a growth factor active in angiogenesis, stimulating endothelial cell proliferation and migration.
• PlGF binds to the receptor FLT1/VEGFR-1, mediating downstream signaling in endothelial cells.
• It promotes cell tumor growth, as supported by similarity annotations.
• The protein is secreted and contains a signal peptide, consistent with its extracellular role in intercellular signaling.
• PlGF is classified under keywords including angiogenesis, developmental protein, differentiation, disulfide bond, glycoprotein, growth factor, mitogen, and secreted.
• The predicted molecular weight is approximately 17.9 kDa (based on the mature protein after signal peptide cleavage), aligning with the observed 17 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 24–158) corresponds to the predicted mature mouse PlGF, likely enabling recognition of both precursor and processed forms.
• For Western blotting, a band around 17 kDa may be detected under reducing conditions; consider optimizing sample preparation and antibody dilution.
• In ELISA applications, this antibody may serve as a capture or detection reagent; pairing with a matched antibody pair could enhance sensitivity.
• As a polyclonal antibody, lot-to-lot variability is inherent; validate and titrate the antibody in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw material partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For angiogenesis and growth factor research, we support sourcing of rare target raw materials including PlGF-related products and provide technical consultation to accelerate diagnostic development from concept to clinic.