Anti-Mouse PD-L2/CD273/B7-DC Monoclonal Antibody () for Flow Cytometry - Q9WUL5

Core Product Specifications and Parameters

Biological Background: PD-L2/CD273/B7-DC Function and Localization

• PD-L2 (also known as B7-DC, CD273) is a member of the B7 family of immune regulatory ligands, encoded by the Pdcd1lg2 gene.
• It serves as a ligand for the inhibitory receptor PD-1 (PDCD1), delivering a critical inhibitory signal that maintains peripheral tolerance and prevents autoimmunity. Related references: PMID:11224527 | PMID:11283156 | PMID:12719480
• Upon binding to PD-1, PD-L2 inhibits T-cell proliferation by blocking cell cycle progression and reducing cytokine production. Related references: PMID:11224527 | PMID:11283156 | PMID:12719480
• PD-L2 is a transmembrane glycoprotein localized on the cell surface, featuring immunoglobulin-like domains and a signal peptide.
• It is highly expressed in dendritic cells, placenta, and liver, with lower expression in heart, spleen, lymph nodes, and thymus; also found on some lymphoid tumor cell lines.
• Post-translational modifications, including glycosylation and disulfide bond formation, are essential for its structural integrity and ligand-receptor interaction.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , eliminating the need for a secondary antibody and streamlining multi-color flow cytometry panels.
• Titrate the antibody to determine the optimal working concentration for your specific cell type and assay conditions; as a reference, 0.5–5 µg per test is a common starting range, but empirical validation is essential.
• Use a mouse dendritic cell line or appropriately stimulated primary dendritic cells as a positive control, and include an isotype control to assess non-specific binding.
• For optimal membrane staining, process live cells on ice, wash thoroughly, and avoid harsh fixation that may alter epitope recognition.
• Validate performance in your own experimental system, as specific staining may vary with cell activation state and PD-L2 expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and labs to provide one-stop access to IVD raw materials, including validated monoclonal antibodies like this anti-PD-L2 clone for immune checkpoint research. Our portfolio encompasses optimized antibody pairs, recombinant proteins, and ancillary reagents, supporting immunology and oncology assay development. We help streamline the sourcing of rare and high-quality raw materials—contact us for bulk requests or custom conjugation needs.