Anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for Flow Cytometry - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• The product targets mouse MHC Class II molecules, specifically the I-A and I-E isotypes, which are encoded by multiple genes: H2-Aa, H2-Ab, H2-Eb1, and H2-Eb2.
• MHC class II molecules are heterodimers consisting of an alpha and a beta chain, and they play a central role in adaptive immunity by presenting processed extracellular antigens to CD4+ T cells.
• The I-A/I-E proteins are type I membrane glycoproteins containing disulfide bonds and are subject to ubiquitin conjugation, as indicated by UniProt keywords.
• Subcellular localization is primarily at the plasma membrane, where they function in antigen presentation.
• According to UniProt (P18468), the I-E beta chain has a predicted molecular weight of ~30 kDa; however, the observed protein weights may vary due to glycosylation and other post-translational modifications.
• Keywords from UniProt: Adaptive immunity, Disulfide bond, Glycoprotein, Immunity, Membrane, MHC II, Signal, Transmembrane helix, Ubl conjugation.

Experimental Guidance and Technical Tips

• For flow cytometry, perform antibody titration to determine the optimal working concentration for your cell population.
• The recombinant protein immunogen suggests that this antibody may recognize both native and denatured forms of MHC class II, but validation in each application is recommended.
• Include appropriate isotype controls and compensation controls when using fluorescently conjugated antibodies in multicolor panels.
• Since MHC class II expression can be upregulated by activation or cytokine stimulation, consider stimulating cells to enhance detection if baseline expression is low.
• Store the antibody as recommended, avoiding repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, from validated antibody pairs and bulk monoclonal antibodies to ancillary reagents, supporting immunology and histocompatibility research. With this anti-MHC Class II antibody, we provide a reliable tool for flow cytometry-based diagnostic assays. Our sourcing services ensure rapid access to rare and custom raw materials, accelerating your development from concept to clinic.