Anti-Mouse Ly-6A/E (Sca-1) Monoclonal Antibody for FC - P05533

Core Product Specifications and Parameters

Biological Background: Ly-6A/E (Sca-1) Function and Localization

• Lymphocyte antigen 6A-2/6E-1, also known as stem cell antigen 1 (Sca-1) or T-cell-activating protein (TAP), is a member of the Ly-6/uPAR superfamily of GPI-anchored cell surface proteins.
• The primary function of Ly-6A/E is T-cell activation, playing a role in lymphocyte signaling.
• Encoded by the Ly6a (Ly6) gene in mice, this protein is widely expressed across various tissues.
• Ly-6A/E is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage, localizing it to lipid rafts.
• The protein undergoes post-translational modifications including N-linked glycosylation and disulfide bond formation, which are critical for its structure and function.
• As a GPI-anchored lipoprotein, Ly-6A/E is involved in membrane microdomain organization and signal transduction.
• The predicted molecular weight of Ly-6A/E is approximately 14.4 kDa, based on its amino acid sequence.

Experimental Guidance and Technical Tips

• This antibody is supplied as a direct conjugate to , enabling one-step staining for flow cytometry without a secondary antibody.
• For optimal flow cytometry staining, titrate the antibody to determine the best concentration for your specific cell type and protocol.
• Since Ly-6A/E is a GPI-anchored membrane protein, permeabilization is not required for surface staining; however, ensure cells are viable and properly blocked to reduce non-specific binding.
• The immunogen corresponds to the extracellular domain (amino acids 27-111), so the antibody is expected to recognize the native surface protein.

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