Anti-Mouse Integrin Alpha 1/CD49a Monoclonal Antibody () for Flow Cytometry - Q3V3R4

Core Product Specifications and Parameters

Biological Background: Integrin Alpha-1 Function and Localization

• Integrin alpha-1 (CD49a) is the alpha subunit of the alpha-1/beta-1 integrin complex, also known as very late antigen-1 (VLA-1), which functions as a receptor for extracellular matrix proteins.
• Specifically recognizes and binds to laminin and collagen via a proline-hydroxylated G-F-P-G-E-R motif, mediating cell-matrix adhesion.
• Plays a role in anchorage-dependent negative regulation of epidermal growth factor (EGF)-stimulated cell growth, suggesting involvement in growth control signaling.
• It is a single-pass type I membrane protein localized to the plasma membrane.
• Contains multiple functional domains including calcium- and magnesium-binding sites, disulfide bonds, and glycosylation sites, typical of integrin family members.
• Predicted molecular weight of the unmodified precursor is approximately 130.8 kDa.

Experimental Guidance and Technical Tips

• This antibody is provided as an conjugate, enabling direct detection by flow cytometry without the need for secondary staining, which reduces background and simplifies the workflow.
• For flow cytometry, prepare a single-cell suspension from the relevant mouse tissue or cell line and titrate the antibody to determine the optimal concentration for specific and bright staining.
• Include appropriate controls such as unstained cells, isotype control antibody conjugated to the same fluorophore, and compensation controls when performing multicolor flow cytometry.
• Since the immunogen spans nearly the entire extracellular domain (aa 29-1141), the antibody likely recognizes native epitopes; however, confirm reactivity in the specific biological context of interest.
• Cell surface expression of integrin alpha-1 may be modulated by activation status, so cell culture conditions and stimulation protocols should be carefully standardized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers and research laboratories. For integrin alpha-1 research and assay development, we offer a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your workflows. Our team can also assist in sourcing rare-target raw materials and providing technical consultation to accelerate your project from concept to commercialization.