Anti-Mouse IL4 Monoclonal Antibody (APC) for FC - P07750

Core Product Specifications and Parameters

Biological Background: IL4 Function and Localization

• Interleukin-4 (IL4, also known as B-cell stimulatory factor 1/BSF-1) is a secreted cytokine that plays a key role in regulating antibody production, hematopoiesis, inflammation, and the development of effector T-cell responses. Related references: PMID:3083412
• IL4 induces the expression of class II MHC molecules on resting B-cells and enhances both secretion and cell surface expression of IgE and IgG1. Related references: PMID:3498301
• IL4 signaling occurs through two receptor complexes: type 1 (IL4R and common gamma chain) mainly on hematopoietic cells, and type 2 (IL4R and IL13RA1) on nonhematopoietic cells, leading to JAK/STAT6 pathway activation. Related references: PMID:25847241, PMID:8624821
• Positively regulates IL31RA expression in macrophages and stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and inducing RUFY4. Related references: PMID:26416964
• Plays a critical role in higher brain functions, including memory and learning. Related references: PMID:25772794, PMID:28202615
• Secreted protein with a molecular weight of ~16 kDa; contains disulfide bonds and is glycosylated.
• Also known as B-cell IgG differentiation factor, B-cell growth factor 1, IGG1 induction factor, and lymphocyte stimulatory factor 1.
• Gene: Il4 (Il-4) located on chromosome 11 in mouse.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry (FC intra), cells must be properly fixed and permeabilized to allow antibody access to the secreted IL4. Use of protein transport inhibitors (e.g., brefeldin A) may be required to accumulate intracellular cytokine.
• The immunogen corresponds to amino acids 21-140 of mouse IL4 (NP_067258.1). Validate antibody specificity using IL4 knockout mouse cells or by pre-incubating with recombinant mouse IL4 protein as a blocking control.
• This antibody is intended for mouse samples; cross-reactivity with other species has not been tested. Consider confirming performance in your experimental system with appropriate positive (e.g., activated Th2 cells) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for sourcing rare targets. For the IL4 research area, CamelBio offers a range of cytometry-grade antibodies and protein raw materials to accelerate your assay development.