Anti-Mouse IL1R1/CD121a Monoclonal Antibody () for Flow Cytometry - P13504

Core Product Specifications and Parameters

Biological Background: Interleukin-1 Receptor Type 1 Function and Localization

• Interleukin-1 receptor type 1 (IL-1R1, CD121a) is a transmembrane receptor encoded by the Il1r1 gene, and serves as the primary receptor for interleukin-1 (IL-1) cytokines IL1A, IL1B, and the IL-1 receptor antagonist IL1RN.
• Upon ligation, IL-1R1 associates with the coreceptor IL1RAP to form a high-affinity signaling complex, triggering downstream NF-kappa-B and MAPK pathways critical for inflammatory and immune responses.
• The receptor's intracellular TIR domain recruits adaptors like MYD88 and IRAKs, leading to kinase cascades; IL-1R1 also participates in IL1B-mediated costimulation of IFNG production in Th1 cells.
• Isoform 2, generated by alternative promoter usage, cannot mediate canonical IL-1 signaling but instead cooperates with IL1RAP isoform 3 to modulate neuronal activity, including NMDA-induced calcium influx via Akt.
• IL-1R1 is primarily localized on the cell membrane and can also be found as a secreted form; isoform 2 is specifically expressed in various brain tissues.
• The protein features extracellular immunoglobulin-like domains, multiple disulfide bonds, glycosylation, and phosphorylation sites, consistent with its role as a transmembrane signaling receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20–338), making this antibody well-suited for detecting cell surface IL-1R1 by flow cytometry.
• For flow cytometry, use appropriate isotype controls and titrate the antibody to optimize signal-to-noise ratio; consider fixation/permeabilization protocols if intracellular staining is desired.
• Verify specificity using IL-1R1 knockout cell lines or by pre-blocking with recombinant mouse IL-1R1 protein.
• The predicted molecular weight is 67 kDa; however, post-translational modifications such as glycosylation may result in a higher apparent molecular weight on SDS-PAGE.
• This antibody recognizes mouse IL-1R1; cross-reactivity with other species has not been reported and should be validated independently.

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