Anti-Mouse IL-33R/ST2 Monoclonal Antibody () for Flow Cytometry - P14719

Core Product Specifications and Parameters

Biological Background: IL-33R/ST2 Function and Localization

• IL-33R/ST2, encoded by the Il1rl1 gene, is a member of the interleukin-1 receptor family and serves as the receptor for interleukin-33 (IL-33), playing critical roles in innate and adaptive immunity. Related references: PMID:17675517 | PMID:18450470 | PMID:22660580
• Upon IL-33 binding and complex formation with coreceptor IL1RAP, ST2 recruits MYD88, IRAKs, and TRAF6, leading to downstream MAPK pathway activation and modulation of immune responses.
• Alternatively spliced isoforms exist, including transmembrane (ST2L) and soluble (sST2) forms, which can act as a decoy receptor inhibiting IL-33 signaling.
• ST2 is predominantly expressed in hematopoietic tissues and inflammatory cell types such as macrophages, erythroid cells, epithelial cells, and fibroblasts, with isoform-specific distribution in brain astrocytes, microglia, and endothelial cells.
• The protein undergoes post-translational modifications including glycosylation, phosphorylation, and ubiquitination, and contains immunoglobulin-like domains and disulfide bonds.
• In macrophages, ST2 engagement during tissue injury can trigger UCP2-dependent mitochondrial rewiring, attenuating ROS generation and promoting an anti-inflammatory phenotype. Related references: PMID:34644537

Experimental Guidance and Technical Tips

• The immunogen, covering amino acids 27–332 of the mouse ST2 extracellular domain, is likely to recognize both membrane-bound and soluble isoforms; consider validation in the relevant sample system (e.g., cell lines, tissue homogenates) to confirm isoform detection.
• For flow cytometry applications, titrate the antibody to determine the optimal staining concentration for your cell type of interest, and include appropriate isotype controls.
• Verify cross-reactivity in your mouse model as expression levels may vary; testing on knockout or knockdown samples is recommended to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides customized IVD raw material solutions, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for IL-33/ST2 research. Whether you require rare-target antibodies or a complete sourcing strategy from concept to clinic, our integrated supply chain and technical expertise support your entire development workflow.