Anti-Mouse IL-33R/ST2 Monoclonal Antibody for Flow Cytometry - P14719

Core Product Specifications and Parameters

Biological Background: IL-33R/ST2 Function and Localization

• IL-33R/ST2 (Interleukin-1 receptor-like 1) is the receptor for interleukin-33 (IL-33), playing essential roles in innate and adaptive immunity, tissue homeostasis, and responses to environmental stresses. Related references: PMID:17675517, PMID:18450470, PMID:22660580
• Upon IL-33 binding, ST2 recruits the coreceptor IL1RAP and downstream adaptors MYD88, IRAK1/4, and TRAF6, leading to phosphorylation of MAP kinases ERK1/2 and p38 MAPK.
• ST2 signaling induces UCP2-dependent mitochondrial rewiring upon tissue injury, attenuating reactive oxygen species generation and preserving Krebs cycle integrity to promote itaconate production and GATA3-dependent differentiation of anti-inflammatory macrophages. Related references: PMID:34644537
• The receptor exists as multiple isoforms via alternative splicing: a long membrane-bound form (ST2L) and a soluble secreted form (sST2), which acts as a decoy inhibitor of IL-33 signaling.
• ST2 is predominantly expressed in hematopoietic tissues, including macrophage, erythroid, epithelial, and fibroblast cell lines; isoform A is found in brain astrocytes and microglia, isoform B in brain endothelial cells.
• The protein contains immunoglobulin-like domains, disulfide bonds, and glycosylation sites, and is subject to ubiquitination and phosphorylation.
• Possibly involved in helper T-cell function.

Experimental Guidance and Technical Tips

• Since this antibody is directly conjugated to , no secondary detection step is required for flow cytometry.
• The immunogen corresponds to the extracellular domain of mouse ST2 (aa 27-332), so the antibody is expected to recognize the cell surface receptor; consider validating in appropriate mouse cell lines or primary cells known to express ST2, such as macrophages or T cells.
• Titrate the antibody to determine optimal concentration for your specific cell type and experimental conditions.
• Note that both membrane-bound and soluble forms exist; for intracellular staining, permeabilization may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one‑stop access to IVD raw materials, technical services, and consulting—from concept to clinic. For studies targeting IL-33R/ST2 and IL-33 signaling in innate/adaptive immunity or inflammation, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and help source rare-target raw materials to accelerate your assay development.