Anti-Mouse IL-12 p40 Monoclonal Antibody () for Intracellular Flow Cytometry - P43432

Core Product Specifications and Parameters

Biological Background: IL-12 p40 Function and Localization

• IL-12 p40 is a subunit of interleukin-12 (IL-12) and interleukin-23 (IL-23), central cytokines in immune regulation.
• In IL-12, it pairs with p35 to form the biologically active IL-12p70, which promotes Th1 differentiation and IFN-γ production.
• As a component of IL-23, p40 partners with p19 (IL23A) to drive Th17 responses and pro-inflammatory cytokine release.
• IL-12 p40 acts as a growth factor for activated T and NK cells, enhancing their lytic activity and stimulating IFN-γ secretion by resting PBMCs.
• Its signaling through the IL-12 receptor activates the Jak-Stat pathway, linking innate and adaptive immunity.
• It is a secreted glycoprotein with a signal peptide, immunoglobulin-like domain, and disulfide bonds, reflecting its cytokine structure.
• The mouse Il12b gene (Gene ID: 16160) encodes a 335-amino acid precursor, with the mature protein weight around 38 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23–335 of mouse IL-12 p40, covering most of the extracellular domain; the antibody may detect both the free subunit and p40 within IL-12/IL-23 heterodimers.
• For intracellular flow cytometry, optimize permeabilization conditions and titrate the antibody to determine the optimal staining concentration.
• Include appropriate isotype controls and use activated mouse immune cells (e.g., LPS/IFN-γ-stimulated macrophages or dendritic cells) as positive controls.
• Because IL-12 p40 is secreted, a protein transport inhibitor (e.g., brefeldin A) may be required to retain the cytokine intracellularly prior to staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined one-stop channel for IVD raw-material procurement, providing production-grade antibodies and ancillary reagents to support diagnostic development. For IL-12 research, we supply validated antibody pairs, optimized monoclonal antibodies (including fluorophore-conjugated formats), and bulk reagents tailored to immunoassay platforms. Our team also assists with sourcing rare-target raw materials and technical consultation, bridging your project from concept to clinic.