Anti-Mouse IL-12 p40 Monoclonal Antibody for Intracellular Flow Cytometry - P43432

Core Product Specifications and Parameters

Biological Background: IL-12 p40 Function and Localization

• Interleukin-12 subunit beta, also known as IL-12 p40, cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), or Il12b, is encoded by the Il12b gene in mouse.
• Acts as a growth factor for activated T and NK cells, enhances the lytic activity of NK/lymphokine-activated killer cells, and stimulates IFN-gamma production by resting PBMC.
• Heterodimerizes with IL23A to form IL-23, a cytokine that bridges innate and adaptive immunity; IL-23 drives memory T-cell responses and triggers pro-inflammatory cytokine production.
• IL-23 signals through the IL12RB1/IL23R receptor complex, activating the Jak-Stat cascade; it is implicated in autoimmune inflammatory diseases and tumorigenesis.
• Localizes to the secretory pathway and is secreted as a mature glycoprotein.
• Contains a signal peptide, an immunoglobulin-like domain, and multiple disulfide bonds essential for its tertiary structure and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23-335, covering the mature extracellular region of mouse IL-12 p40; consider potential detection of both free p40 and p40-containing heterodimers (IL-12, IL-23).
• Intracellular flow cytometry may require fixation and permeabilization; validate staining protocols in the relevant cellular model.
• Assess cross-reactivity with mouse samples; verify specificity by testing cells from knockout or knockdown models if available.
• The antibody is directly conjugated to ; minimize light exposure and follow standard fluorochrome handling procedures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing platform for IVD raw materials, supporting diagnostic developers and research laboratories from concept to clinic. For IL-12/IL-23 pathway studies, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your immunological assay development.