Anti-Mouse IFN-beta Rabbit Monoclonal Antibody for WB and ELISA - P01575

Core Product Specifications and Parameters

Biological Background: IFN-beta Function and Localization

• Interferon beta (IFN-beta) is a type I interferon cytokine that orchestrates innate immune responses against viral and bacterial infections, as well as developing tumors. Related references: PMID:10708458 PMID:23872679
• It signals primarily through the heterodimeric receptor IFNAR1/IFNAR2, activating the JAK-STAT pathway to induce transcription of interferon-regulated genes involved in antiviral defense, cell proliferation, and immunoregulation. Related references: PMID:23872679
• IFN-beta is more potent than IFN-alpha in triggering apoptotic and antiproliferative pathways that control tumor cell growth. Related references: PMID:14597717
• It plays a role in B-cell development, myelopoiesis, and lipopolysaccharide-induced tumor necrosis factor production, contributing to broad immune regulation. Related references: PMID:10708458 PMID:14597717
• In the nervous system, IFN-beta maintains neuronal homeostasis by promoting autophagy and clearance of alpha-synuclein, protecting dopaminergic neurons from degeneration. Related references: PMID:26451483
• The protein is secreted and structurally contains a disulfide bond, glycosylation, and phosphorylation sites, as indicated by its keywords.
• Alternative names for IFN-beta include Ifb, IFNB, and If1da1.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein spanning amino acids 1-182 of mouse IFN-beta (full-length mature protein). This design suggests the antibody may recognize both native and recombinant protein; we recommend validating reactivity in the relevant experimental system.
• For Western blotting, the predicted molecular weight is approximately 22 kDa. Consider using non-reducing sample buffer if disulfide bond preservation is important for epitope recognition.
• For ELISA applications, the antibody can be explored as either a capture or detection reagent. Pairing compatibility with other IFN-beta antibodies should be empirically determined.
• As a rabbit monoclonal antibody, it typically offers high specificity and lot-to-lot consistency; nonetheless, optimal working dilutions should be titrated for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined one-stop platform for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For innate immunity and cytokine research, we offer extensive solutions targeting key molecules such as IFN-beta. Whether you require rare target reagents or comprehensive sourcing support, CamelBio bridges the gap from concept to clinical application.