Anti-Mouse H2-Ab1 Rabbit Monoclonal Antibody for WB - P06345

Core Product Specifications and Parameters

Biological Background: H2-Ab1 (H-2 class II) Function and Localization

• H2-Ab1 encodes the beta chain of the mouse H-2 class II histocompatibility antigen (MHC class II), a key component of the adaptive immune system.
• MHC class II molecules are expressed on antigen-presenting cells and present processed foreign antigens to CD4+ T cells, initiating immune responses.
• The protein contains a signal peptide, extracellular domain with a disulfide bond, transmembrane helix, and a cytoplasmic tail, characteristic of type I membrane proteins.
• It localizes to the cell membrane and endosomal compartments, where it binds and presents peptide antigens.
• Post-translational modifications include glycosylation and ubiquitin-like (Ubl) conjugation, which may regulate expression and turnover.
• Keywords associated with H2-Ab1 include adaptive immunity, disulfide bond, glycoprotein, immunity, membrane, MHC II, signal, transmembrane, transmembrane helix, and Ubl conjugation, reflecting its structural and functional roles.
• The molecular weight is approximately 30 kDa (predicted 29.8 kDa), consistent with the glycosylated mature protein.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 31-219), likely excluding the signal peptide, which may enhance detection of mature MHC class II on the cell surface.
• For Western blot (WB), expect a band around 30 kDa; consider using reducing conditions to resolve the monomeric form.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate antibody specificity using knockout tissues or blocking peptides.
• For ELISA, use recombinant protein as a positive control to establish a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive sourcing bridge for IVD raw materials. For targets like H2-Ab1 involved in adaptive immunity, we offer validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents in bulk. Our global supply chain ensures rapid access to rare and specialized targets to accelerate your assay development.