Anti-Mouse H2-Aa Rabbit Polyclonal Antibody for WB and ELISA - P14434

Core Product Specifications and Parameters

Biological Background: H2-Aa Function and Localization

• The H2-Aa gene encodes the alpha chain of the mouse H-2 class II histocompatibility antigen (MHC II), a critical component of the adaptive immune system.
• As an MHC class II molecule, it presents processed extracellular antigens to CD4+ T helper cells, initiating specific immune responses.
• The protein is a single‑pass type I membrane protein with an N‑terminal signal peptide (cleaved in the mature form) and a C‑terminal transmembrane helix, anchoring it in the cell membrane.
• Its extracellular domain contains a disulfide bond and predicted N‑glycosylation sites, contributing to structural stability and proper folding.
• UniProt keywords include Adaptive immunity, Disulfide bond, Glycoprotein, Membrane, MHC II, Signal, and Transmembrane.
• The 3D structure of the protein has been resolved, providing insights into MHC II peptide‑binding and T‑cell receptor interactions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 27–208) covers the entire extracellular domain after signal peptide cleavage, making the antibody suitable for detecting the mature, surface‑expressed H2‑Aa protein.
• For Western blotting, the predicted molecular weight is 28 kDa; however, glycosylation may result in a higher apparent molecular weight. Validate in your sample system with appropriate controls.
• In ELISA, this polyclonal antibody can be used for direct or indirect detection of recombinant or native H2‑Aa, with cross‑reactivity to human MHC II alpha chain potentially observed.
• When applying to human samples, confirm cross‑reactivity and specificity under your experimental conditions, as the antibody is raised against mouse protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting—from concept to clinic. For immunology and MHC‑related research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing for rare‑target raw materials, ensuring a reliable supply chain for your diagnostic or research projects.