Anti-Mouse G-CSF Rabbit Monoclonal Antibody for WB, ELISA - P09920

Core Product Specifications and Parameters

Biological Background: Mouse G-CSF Function and Localization

• Granulocyte colony-stimulating factor (G-CSF), encoded by the Csf3 gene in mice, is a secreted glycoprotein that functions as a hematopoietic growth factor.
• It specifically stimulates the proliferation, differentiation, and functional activation of granulocytes from bone marrow progenitors.
• This cytokine belongs to the granulocyte/macrophage colony-stimulating factor family, which controls the production of granulocytes and monocytes-macrophages.
• Synthesized as a precursor with a signal peptide, mature G-CSF is secreted into the extracellular space to exert systemic effects on hematopoiesis.
• It contains a disulfide bond and undergoes glycosylation, contributing to its structural stability and bioactivity.
• The protein's molecular weight is approximately 22 kDa, as confirmed by direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen covers the mature mouse G-CSF sequence (amino acids 31-208), which may support recognition of both native and denatured protein in Western blot and ELISA.
• For Western blot, a band around 22 kDa is expected; reducing conditions may help resolve monomeric G-CSF from potential oligomers.
• In ELISA, consider pairing this antibody with a matched detection antibody specific for a different epitope to develop a sandwich assay.
• Validate the antibody in your specific sample system, as glycosylation or endogenous binding proteins could influence reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop access to premium IVD raw materials, including validated monoclonal antibodies like this anti-G-CSF antibody. Whether you require antibody pairs, recombinant proteins, or ancillary reagents for hematopoiesis or cytokine research, our comprehensive catalog and sourcing expertise support your projects from concept to clinic.