Anti-Mouse F4/80 Monoclonal Antibody (Biotin) for Flow Cytometry - Q61549

Core Product Specifications and Parameters

Biological Background: F4/80 Function and Localization

• F4/80 (Adgre1, Emr1) is the mouse adhesion G protein-coupled receptor E1, a cell surface glycoprotein and member of the EGF-TM7 family of class B GPCRs.
• It is an orphan receptor involved in cell adhesion and cell-cell interactions, particularly within the immune system; it may also play a role in regulatory T-cell (Treg) development.
• In mice, F4/80 is expressed on macrophages but absent from those localized within T-cell areas of lymph nodes and spleen; it shows low-level expression on blood monocytes, making it a well-established pan-macrophage marker.
• The protein is a multi-pass transmembrane receptor located at the cell membrane.
• Structurally, it contains EGF-like domains and a GPCR autoproteolysis-inducing (GAIN) domain; it is predicted to contain multiple disulfide bonds and glycosylation sites.
• Keywords assigned by UniProtKB include: adaptive immunity, calcium signaling (typical of GPCRs), cell membrane, glycoprotein, and receptor activity.

Experimental Guidance and Technical Tips

• For flow cytometry, optimize the antibody concentration by titration using the target cell population; typical starting dilutions for biotin conjugates range widely and must be determined experimentally.
• The biotin label enables flexible detection with streptavidin-fluorophore conjugates; ensure that the detection reagent is compatible with your panel and laser configuration.
• When staining mouse macrophages, pre-incubate with an Fc receptor block (e.g., anti-CD16/CD32) to reduce nonspecific binding.
• F4/80 is a large transmembrane protein; if fixing and permeabilizing cells, validate that your protocol preserves surface epitope recognition.
• As F4/80 expression is absent from macrophages in T-cell areas of lymphoid tissues, include appropriate positive (e.g., splenic red pulp macrophages) and negative (e.g., lymph node T-cell zone) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality reagents for immune cell profiling. In addition to this biotinylated anti-mouse F4/80 antibody for flow cytometry, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to streamline your supply chain from concept to clinic.