Anti-Mouse CXCR2/IL-8RB Monoclonal Antibody () for Flow Cytometry - P35343

Core Product Specifications and Parameters

Biological Background: CXCR2 Function and Localization

• CXCR2, also known as IL-8 receptor B, GRO/MGSA receptor, or CD128, is a member of the G protein-coupled receptor (GPCR) family, characterized by seven transmembrane domains.
• It serves as a high-affinity receptor for interleukin-8 (IL-8) and related chemokines such as CXCL3, GRO/MGSA, and NAP-2, playing a central role in neutrophil activation and chemotaxis via a G protein-mediated phosphatidylinositol-calcium second messenger system.
• CXCR2 is involved in homeostatic wound healing, guiding neutrophil migration to necrotic sites while preventing collateral damage to healthy tissues. Related references: PMID:20947763
• The receptor is localized to the cell membrane, consistent with its function in transducing extracellular chemokine signals.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation, which are critical for receptor stability, ligand binding, and signal transduction.
• The mouse CXCR2 protein has a calculated molecular weight of approximately 40 kDa (UniProt: 40425 Da), consistent with the observed band in immunochemical assays.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–44) of mouse CXCR2, suggesting that the antibody may recognize an extracellular epitope suitable for live-cell flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions. Include appropriate isotype controls and fluorescence minus one (FMO) controls.
• Consider validating the antibody’s specificity in knockout or knockdown models, or via blocking with the immunogen peptide, to confirm target binding.
• Given the receptor’s role in neutrophil chemotaxis, functional assays (e.g., migration or calcium flux) can be used in parallel to correlate surface receptor expression with biological activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to an extensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For inflammation and chemokine receptor research, we support bulk procurement and custom sourcing of rare targets like CXCR2, enabling accelerated assay development from concept to clinic.