Anti-Mouse CX3CR1 Monoclonal Antibody for Flow Cytometry - Q9Z0D9

Core Product Specifications and Parameters

Biological Background: CX3CR1 Function and Localization

• CX3CR1 (CX3C chemokine receptor 1) is a G protein-coupled receptor that binds fractalkine (CX3CL1), mediating leukocyte adhesion, migration, and survival. Related references: PMID:10187784, PMID:9918795, PMID:11544273, PMID:12871640, PMID:16675847
• It plays a key role in recruiting natural killer cells to inflamed tissues and regulating monocyte-driven inflammation in atherosclerosis and airway disease. Related references: PMID:11544273, PMID:16675847, PMID:12569158, PMID:18971423, PMID:21037587
• In the central nervous system, CX3CR1 is highly expressed on microglia and is essential for synaptic pruning during postnatal development and for restraining neuroinflammatory responses. Related references: PMID:16732273, PMID:21778362, PMID:24487234
• CX3CR1 contributes to intestinal immune surveillance by enabling lamina propria dendritic cells to capture bacteria and by orchestrating antifungal IgG responses against commensal fungi. Related references: PMID:15653504, PMID:29326275, PMID:33548172
• The receptor also negatively regulates angiogenesis via macrophage chemotaxis and can bind the CC chemokine CCL26. Related references: PMID:18322241
• CX3CR1 is a membrane protein found on the surface of leukocyte subsets, including monocytes, dendritic cells, T cells, and NK cells, with expression levels defining functional monocyte subsets. Related references: PMID:10805752, PMID:12871640, PMID:16732273
• Post-translational modifications include phosphorylation, and the protein is classified as a phosphoprotein and member of the GPCR transmembrane receptor family.

Experimental Guidance and Technical Tips

• This antibody is optimized for flow cytometry. Titration on relevant mouse cell populations (e.g., blood leukocytes, splenocytes, or brain microglia) is recommended to determine the optimal working concentration.
• As CX3CR1 is a cell surface receptor, staining can be performed without permeabilization. Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Validate antibody performance in the sample system of interest, as target expression levels may vary depending on activation state and tissue microenvironment.
• For multi-color flow cytometry panels, ensure fluorophore compatibility and compensation controls are in place.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research laboratories a streamlined procurement platform for IVD-grade antibodies, antigens, and ancillary reagents. This CX3CR1 monoclonal antibody is part of our expanding portfolio of immune-related tools, supporting projects in inflammation, neuroscience, and infectious disease. Backed by rigorous quality control and flexible bulk supply options, CamelBio is your reliable partner from concept to clinic.