ABflo 647 Anti-Mouse CD98 (SLC3A2) Monoclonal Antibody for Flow Cytometry - P10852

Core Product Specifications and Parameters

Biological Background: CD98 (SLC3A2) Function and Localization

• CD98 heavy chain, also known as SLC3A2 or 4F2hc, is a type II transmembrane glycoprotein that acts as a chaperone for amino acid transporter light chains of the SLC7 family, forming functional heterodimers essential for amino acid transport (PubMed:10574970, PubMed:10734121, PubMed:11011012). Related references: PMID:10574970 PMID:10734121 PMID:11011012
• These heterodimers, such as SLC3A2/SLC7A5 (LAT1), mediate the cellular uptake of large neutral amino acids including leucine, which in turn activates mTORC1 signaling upon lysosomal recruitment by LAPTM4B.
• SLC3A2 modulates integrin signaling and is crucial for integrin-dependent cell spreading, migration, and tumor progression (By similarity).
• The protein is broadly expressed in mouse tissues, with highest levels in kidney, small intestine, spleen, thymus, and liver; it is also detected on brain capillary endothelia and embryonic epithelia (PubMed:9915839). Related references: PMID:9915839
• Subcellularly, SLC3A2 localizes to the plasma membrane (both apical and basolateral surfaces), lysosomes, melanosomes, and cell junctions, reflecting its diverse roles in transport and adhesion.
• It undergoes various post-translational modifications including N-glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, and is subject to alternative splicing giving rise to multiple isoforms.

Experimental Guidance and Technical Tips

• Since this antibody is directly conjugated to ABflo 647, no secondary antibody is needed for flow cytometry, reducing background and experimental steps.
• Titrate the antibody to determine the optimal staining concentration for your mouse cell samples, and include appropriate isotype controls and viability dyes.
• The immunogen spans the extracellular domain (amino acids 105-526), so the antibody should recognize the native surface form of CD98 on live cells.
• Validate the antibody in the relevant mouse tissue or cell line, considering that CD98 expression may vary; positive controls could include activated lymphocytes or tumor cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies like this anti-CD98 conjugate, to bulk ancillary reagents and rare target sourcing. Our expert team supports projects from early-stage development through clinical application, ensuring a reliable supply chain and technical consultation for your immunoassay and cell analysis needs. Contact us to discuss how we can assist with your CD98-targeted research or diagnostic panel development.