Anti-Mouse CD95/FAS Monoclonal Antibody for FC - P25446

Core Product Specifications and Parameters

Biological Background: CD95/FAS Function and Localization

• CD95/FAS (also known as Apo-1 antigen, FAS receptor, and TNFRSF6) is a transmembrane receptor of the tumor necrosis factor receptor superfamily that triggers apoptosis upon binding to its ligand FASLG.
• Activation of FAS leads to recruitment of the adapter protein FADD and procaspase-8 to form the death-inducing signaling complex (DISC), where caspase-8 is cleaved and initiates a cascade of effector caspases.
• This apoptotic signaling is essential for peripheral immune tolerance and activation-induced cell death of mature T-cells, maintaining lymphocyte homeostasis.
• FAS is located on the cell membrane and enriched in membrane rafts, with tissue expression detected in thymus, liver, lung, heart, and adult ovary.
• Key post‑translational modifications include glycosylation, palmitoylation, and phosphorylation; the protein also contains a signal peptide, disulfide bonds, a transmembrane domain, and calmodulin-binding sites.
• UniProt annotations classify FAS as an apoptosis receptor with attributes including glycoprotein, lipoprotein, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant mouse CD95/FAS protein; therefore, the antibody primarily recognizes the extracellular domain, making it suitable for surface staining by flow cytometry.
• For FC assays, consider titrating the antibody to determine the optimal concentration for your specific cell type and staining conditions.
• Include appropriate isotype controls and single-stain compensation controls when performing multicolor flow cytometry.
• Validate the antibody’s reactivity in the relevant mouse strain or tissue, as expression levels of FAS can vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies for apoptosis and immunology targets like CD95/FAS. Through our global sourcing network, we offer optimized antibody pairs, bulk ancillary reagents, and tailored procurement support for rare or custom targets. Partner with CamelBio to accelerate your diagnostic development from concept to clinic with reliable, research-grade materials.