Anti-Mouse CD94 Monoclonal Antibody () for Flow Cytometry - O54707

Core Product Specifications and Parameters

Biological Background: CD94 Function and Localization

• Natural killer cells antigen CD94 (Killer cell lectin-like receptor subfamily D member 1) is encoded by the Klrd1 gene in mice and functions as a key immune receptor on NK cells and some T cell subsets.
• CD94 forms heterodimers with NKG2 family members (KLRC1 or KLRC2) to recognize non-classical MHC class Ib molecule MHC-E loaded with self-peptides, enabling self-nonself discrimination by monitoring MHC class I expression.
• The KLRD1-KLRC1 complex acts as an inhibitory receptor, counteracting T cell receptor signaling on memory/effector CD8+ T cells and regulating NK cell activation to prevent autoimmunity; in the tumor microenvironment, it may contribute to immune exhaustion.
• The KLRD1-KLRC2 complex functions as an activating receptor, recognizing MHC-E loaded with MHC-G signal peptides, and plays roles in adaptive NK cell responses, viral infection, and maternal-fetal tolerance.
• As a ligand-binding subunit lacking intrinsic signaling capacity, CD94 relies on partner adaptor proteins (DAP12 for activating signals, or ITIM-mediated phosphatases for inhibitory signals).
• Subcellularly, CD94 is a transmembrane glycoprotein located on the cell membrane, featuring a signal-anchor, a lectin-like domain, disulfide bonds, and glycosylation sites.
• Key biological keywords include adaptive immunity, innate immunity, lectin, immune receptor, and transmembrane signaling, highlighting its central role in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 59-179 of mouse CD94, encompassing the extracellular domain; thus, this antibody may be well-suited for detecting native surface CD94 by flow cytometry.
• For flow cytometry, perform titration experiments to determine the optimal antibody dilution; typical starting concentrations range from 0.5–5 μg/mL, but must be verified in the relevant sample system.
• Include appropriate isotype control (rabbit IgG, conjugate) and Fc receptor blocking to ensure specificity, especially in mouse samples with high FcγR expression.
• Validate antibody performance in positive and negative control samples, such as mouse NK cell lines (e.g., NK-92) or primary splenocytes from wild-type versus CD94 knockout mice.
• Avoid repeated freeze-thaw cycles; store the antibody undiluted at 2–8°C protected from light for short-term, or aliquot and store at -20°C or lower for long-term storage.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement with a comprehensive portfolio covering validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For immune checkpoint and NK cell research applications like CD94, we offer reliable supply solutions to accelerate your diagnostic development from concept to clinic.